3OP7

Crystal structure of a PLP-dependent aminotransferase (ZP_03625122.1) from Streptococcus suis 89-1591 at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-23
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	97.518, 97.518, 89.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.748 - 1.700
R-factor	0.1653
Rwork	0.164
R-free	0.19740
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.671
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.748	29.748	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge		0.053	0.920
Total number of observations		3822	25552
Number of reflections	47906
<I/σ(I)>	11.2	11.7	0.8
Completeness [%]	100.0	97.1	100
Redundancy	7.3	6.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.20M lithium sulfate, 2.00M ammonium sulfate, Additive: 0.001M pyridoxal 5'-phosphate (PLP), NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K