3OP1
Crystal Structure of Macrolide-efflux Protein SP_1110 from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-26 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.153, 73.247, 87.518 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 35.761 - 2.488 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 36456 | |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Lithium sulfate, 0.1 M TRIS pH 8.5 40 %v/v PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
