3OOW
Octameric structure of the phosphoribosylaminoimidazole carboxylase catalytic subunit from Francisella tularensis subsp. tularensis SCHU S4.
Experimental procedure
| Experimental method | MOLECULAR REPLACEMENT |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-13 |
| Detector | MAR CCD |
| Wavelength(s) | 0.75145 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.560, 96.300, 128.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.090 - 1.750 |
| R-factor | 0.17016 |
| Rwork | 0.168 |
| R-free | 0.20510 |
| Structure solution method | SAD |
| Starting model (for MR) | 3opq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.500 |
| Data scaling software | XSCALE |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 110138 | |
| <I/σ(I)> | 12.54 | 3.38 |
| Completeness [%] | 99.0 | 98.8 |
| Redundancy | 7.4 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M NH4 Dihydrogen PO4, 0.1 M Tris, 6% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
