3OOA
Crystal structures and biochemical characterization of the bacterial solute receptor AcbH reveal an unprecedented exclusive substrate preference for b-D-galactopyranose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.250, 66.117, 177.709 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 33.750 - 2.040 |
| R-factor | 0.1704 |
| Rwork | 0.168 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model from SeMet SAD phasing |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.047 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.750 | 2.090 | |
| High resolution limit [Å] | 2.040 | 9.120 | 2.040 |
| Rmerge | 0.104 | 0.026 | 0.538 |
| Rmeas | 0.031 | 0.655 | |
| Number of reflections | 57305 | 689 | 4119 |
| <I/σ(I)> | 9.48 | 25.3 | 2.2 |
| Completeness [%] | 98.4 | 97.5 | 96.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 2.2M (NH4)2SO4, 100mM citric acid , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
