3OO1
Structure of E. Coli CheY mutant A113P in the absence of Sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.240, 52.952, 37.321 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 19.922 - 1.700 |
| R-factor | 0.163 |
| Rwork | 0.160 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3chy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_336)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Number of reflections | 24375 | ||
| <I/σ(I)> | 27.1 | 43.9 | 3.3 |
| Completeness [%] | 98.9 | 98.4 | 87.4 |
| Redundancy | 3.7 | 3.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 28% PEG 8000 100 mM Na Cacodylate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
