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3ONN

Crystal structure of 5'-nucleotidase SDT1 from saccharomyces cerevisiae

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2009-01-02
DetectorMAR scanner 345 mm plate
Wavelength(s)1.54178
Spacegroup nameP 21 21 21
Unit cell lengths40.720, 77.550, 82.160
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution38.775 - 1.870
R-factor0.2004
Rwork0.198
R-free0.24980
Structure solution methodSAD
RMSD bond length0.007
RMSD bond angle1.038
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareSOLVE
Refinement softwarePHENIX ((phenix.refine: 1.6_289))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.7201.960
High resolution limit [Å]1.8601.860
Rmerge0.0710.470
Number of reflections22261
<I/σ(I)>21.24.7
Completeness [%]98.589.8
Redundancy8.98.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.528730% PEG 4000, 0.1M Tris-HCl buffer, pH 8.5, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K

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