3ON4
Crystal structure of TetR transcriptional regulator from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97967 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.380, 124.990, 151.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.190 - 1.850 |
| R-factor | 0.17947 |
| Rwork | 0.179 |
| R-free | 0.21781 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.082 | 0.734 |
| Number of reflections | 130984 | |
| <I/σ(I)> | 21.5 | 2.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 M Li2SO4, 0.1 M Tris/HCl, 40% (w/v) PEG400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
