3OMZ
Crystal structure of MICA-specific human gamma delta T cell receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 |
| Unit cell lengths | 112.370, 112.370, 108.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.830 - 3.040 |
| R-factor | 0.26728 |
| Rwork | 0.265 |
| R-free | 0.30779 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.054 | 0.365 |
| Number of reflections | 25563 | |
| <I/σ(I)> | 48.4 | 2.1 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 0.4M ammonium sulfate, 1.0M lithium sulfate, 0.1M sodium citrate, 0.03M glycyl-glycyl-glycine, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
