3OM1
Crystal structure of the GluK5 (KA2) ATD dimer at 1.7 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.344, 65.635, 113.772 |
| Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
| Resolution | 29.894 - 1.677 |
| R-factor | 0.1696 |
| Rwork | 0.168 |
| R-free | 0.19630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3om0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.677 | 1.680 |
| Rmerge | 0.065 | 0.401 |
| Number of reflections | 91506 | |
| <I/σ(I)> | 18.1 | 2.4 |
| Completeness [%] | 96.6 | 77.3 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1 M BICINE, 20% PEG 8K, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
