3OKA
Crystal structure of Corynebacterium glutamicum PimB' in complex with GDP-Man (triclinic crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.070, 50.020, 85.300 |
| Unit cell angles | 92.05, 92.68, 89.94 |
Refinement procedure
| Resolution | 43.800 - 2.200 |
| R-factor | 0.19097 |
| Rwork | 0.189 |
| R-free | 0.22100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 0.238 |
| Number of reflections | 35191 | |
| <I/σ(I)> | 11.6 | 5.9 |
| Completeness [%] | 95.1 | 81.2 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 6-26% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.1 M lithium sulfate and 0.1 M glycine, VAPOR DIFFUSION, temperature 291K |
