3OK4
Crystal structure of the ANA:RNA decamer suffering from lattice translocation defects
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.220, 34.210, 170.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.460 - 1.149 |
| R-factor | 0.22295 |
| Rwork | 0.221 |
| R-free | 0.25288 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (D/E) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 85.460 | 85.460 | 1.210 |
| High resolution limit [Å] | 1.149 | 3.640 | 1.149 |
| Number of reflections | 70276 | ||
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 97.2 | 93.1 | 95.3 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
