3OIV
H-RasG12V with allosteric switch in the "off" state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.883, 87.883, 133.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.404 - 1.837 |
| R-factor | 0.1621 |
| Rwork | 0.158 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 3.960 | 1.840 |
| Rmerge | 0.131 | 0.049 | 0.643 |
| Number of reflections | 17438 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 99.7 | 99.6 | 97.7 |
| Redundancy | 10.1 | 10.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 7.5 | 298 | 200 Mm Calcium Chloride, 20 % PEG 3350, pH 7.5, sitting drop, temperature 298K |
