3OIN

Crystal structure of Saccharomyces cerevisiae Nep1/Emg1 bound to S-adenosylhomocysteine and 1 molecule of cognate RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00931
Spacegroup name	P 21 21 21
Unit cell lengths	44.774, 88.749, 115.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.680 - 1.900
R-factor	0.1941
Rwork	0.192
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3j
RMSD bond length	0.008
RMSD bond angle	1.069
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.066	0.047	0.392
Number of reflections	36263
<I/σ(I)>	25.5
Completeness [%]	96.8	99	80.2
Redundancy	7.1	7.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 10% PEG 6000, 20% glycerol, pH 7.0, Vapor diffusion, hanging drop, temperature 293K