3OHP
Crystal structure of HGPRT from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.511, 145.213, 51.611 |
| Unit cell angles | 90.00, 94.76, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.040 |
| R-factor | 0.18235 |
| Rwork | 0.180 |
| R-free | 0.22421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9s |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (10.2.23) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.070 | 0.353 |
| Number of reflections | 46597 | |
| <I/σ(I)> | 17.8 | 3.4 |
| Completeness [%] | 99.1 | 92.6 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M citrate PH 5.5, 20% PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
