3OHN
Crystal structure of the FimD translocation domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.022, 87.132, 95.883 |
| Unit cell angles | 63.92, 88.43, 76.94 |
Refinement procedure
| Resolution | 28.890 - 3.011 |
| R-factor | 0.2334 |
| Rwork | 0.230 |
| R-free | 0.30530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (PHENIX) |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.115 | 0.645 |
| Number of reflections | 27375 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 98.4 | 96 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 100mM Trisodium citrate,pH4.8~6.5, 7% (w/v) PEG4000, 100mM sodium chloride, 50mM Magnesium, 20mM spermine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
