3OH1
Protein structure of USP from L. major bound to URIDINE-5'-DIPHOSPHATE-Galacturonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.226, 122.320, 61.221 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 29.509 - 2.180 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.24330 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ogz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 4.700 | 2.180 |
| Rmerge | 0.042 | 0.024 | 0.301 |
| Number of reflections | 39879 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 99.5 | 96.3 | 100 |
| Redundancy | 6.3 | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1-0.2 M sodium tartrate, 16-20% PEG5000, 10 mM DTT, pH 7-8, vapor diffusion, sitting drop, temperature 292K |
