3OGR
Complex structure of beta-galactosidase from Trichoderma reesei with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 67.430, 69.280, 81.500 |
Unit cell angles | 109.03, 97.34, 114.38 |
Refinement procedure
Resolution | 19.780 - 1.500 |
R-factor | 0.1303 |
Rwork | 0.128 |
R-free | 0.16758 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3og2 |
RMSD bond length | 0.025 |
RMSD bond angle | 1.977 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 179614 | |
Completeness [%] | 92.6 | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG 8000, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |