3OGM
Structure of COI1-ASK1 in complex with coronatine and the JAZ1 degron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-07-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.170, 220.758, 149.540 |
| Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
| Resolution | 49.942 - 3.340 |
| R-factor | 0.2266 |
| Rwork | 0.226 |
| R-free | 0.26980 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.410 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Number of reflections | 95997 | |
| Completeness [%] | 92.9 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 1.7M ammonium phosphate, 100mM BTP, 100mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
