3OGC
KcsA E71A variant in presence of Na+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC Q315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | I 4 |
| Unit cell lengths | 154.735, 154.735, 75.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.700 - 3.800 |
| R-factor | 0.24872 |
| Rwork | 0.247 |
| R-free | 0.28289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2atk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.700 | 50.000 | 3.890 |
| High resolution limit [Å] | 3.800 | 9.350 | 3.800 |
| Rmerge | 0.107 | 0.024 | 0.445 |
| Number of reflections | 8872 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.8 | 98.2 | 100 |
| Redundancy | 3.6 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 400, MgAcetate, MES, pH 6.5, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
