3OG2
Native crystal structure of Trichoderma reesei beta-galactosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 |
Unit cell lengths | 67.300, 69.100, 81.500 |
Unit cell angles | 109.10, 97.30, 114.50 |
Refinement procedure
Resolution | 20.000 - 1.200 |
R-factor | 0.1392 |
Rwork | 0.139 |
R-free | 0.17090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tg7 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.300 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 341073 | |
Completeness [%] | 90.6 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 8% PEG 8000, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |