3OF7
The Crystal Structure of Prp20p from Saccharomyces cerevisiae and Its Binding Properties to Gsp1p and Histones
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793, 0.9794, 0.9300 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.510, 75.131, 92.019 |
| Unit cell angles | 90.00, 119.64, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.22420 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.980 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.079 | 0.045 | 0.507 |
| Number of reflections | 71254 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 87.6 | 97.8 | 35.7 |
| Redundancy | 6.9 | 6.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283 | 0.2M tri-Sodium Citrate dihydrate, 15% (w/v) PEG 3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
