3OE7

Structure of four mutant forms of yeast f1 ATPase: gamma-I270T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-15
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	111.030, 292.214, 189.049
Unit cell angles	90.00, 101.82, 90.00

Refinement procedure

Resolution	20.000 - 3.190
R-factor	0.192
Rwork	0.189
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hld
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.190	3.190
Number of reflections	175796
<I/σ(I)>	10.2
Completeness [%]	99.0	98
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.3	277	5.5% PEG 6000, 10% GLYCEROL, 4% METHANOL, 0.05M SODIUM ACETATE, 0.5MM NICKEL SULPHATE, 0.5MM AMP/PNP, 0.025 MM ADP, 2MM MAGNESIUM CHLORIDE, PH 7.3, VAPOR DIFFUSION SITTING DROP, TEMPERATURE 277K