3OE3

Crystal structure of PliC-St, periplasmic lysozyme inhibitor of C-type lysozyme from Salmonella typhimurium

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Wavelength(s)	0.9793, 0.9755
Spacegroup name	C 1 2 1
Unit cell lengths	192.383, 34.685, 74.910
Unit cell angles	90.00, 98.41, 90.00

Refinement procedure

Resolution	19.765 - 1.510
R-factor	0.209
Rwork	0.208
R-free	0.24240
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.970
Data scaling software	SCALA
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.765	19.765	1.590
High resolution limit [Å]	1.510	4.780	1.510
Rmerge		0.071	0.683
Total number of observations		10184	48573
Number of reflections	72824
<I/σ(I)>	8.3	6.4	1
Completeness [%]	94.2	73.7	96.2
Redundancy	5	5.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	26% (w/v) PEG8000, 0.1M imidazole, 0.2M NaCl, pH 7.5, vapor diffusion, hanging drop, temperature 293K