3ODW
Crystal Structure of the Linker-DH/PH domains of p115-RhoGEF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 32 |
| Unit cell lengths | 111.643, 111.643, 97.882 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.670 - 3.200 |
| R-factor | 0.23355 |
| Rwork | 0.231 |
| R-free | 0.28944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3odo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.706 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 22476 | |
| <I/σ(I)> | 14.8 | 2.2 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-26% PEG 3350, 0.2M ammonium sulfate, and 0.1M NaHEPES, pH 7.2-7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
