3OD1
The crystal structure of an ATP phosphoribosyltransferase regulatory subunit/histidyl-tRNA synthetase from Bacillus halodurans C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-22 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97967 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.696, 63.255, 107.637 |
Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
Resolution | 25.677 - 1.970 |
R-factor | 0.1815 |
Rwork | 0.179 |
R-free | 0.22420 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 2.010 |
High resolution limit [Å] | 1.970 | 1.980 |
Rmerge | 0.088 | 0.694 |
Number of reflections | 65055 | |
<I/σ(I)> | 25.3 | 2 |
Completeness [%] | 98.5 | 100 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 12% PEG4000, 0.1M Sodium Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |