3OCO
The crystal structure of a Hemolysin-like protein containing CBS domain of Oenococcus oeni PSU
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924, 0.97943 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 44.454, 93.556, 92.061 |
Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
Resolution | 33.591 - 2.204 |
R-factor | 0.1995 |
Rwork | 0.196 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.122 | 0.377 |
Number of reflections | 17816 | |
<I/σ(I)> | 12.8 | 2.2 |
Completeness [%] | 96.2 | 77.2 |
Redundancy | 3.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 20% PEG3350, 0.2M LiCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |