3OC3

Crystal structure of the Mot1 N-terminal domain in complex with TBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 1 21 1
Unit cell lengths	99.260, 147.820, 103.440
Unit cell angles	90.00, 94.52, 90.00

Refinement procedure

Resolution	47.276 - 3.100
R-factor	0.1899
Rwork	0.187
R-free	0.24210
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	54019
Completeness [%]	99.7	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	50mM Mes pH 6, 200mM Ammoniumacetate, 5% MPD, 4% PEG 3350, 200mM NDSB-201, VAPOR DIFFUSION, HANGING DROP, temperature 291K