3OBY
Crystal structure of Archaeoglobus fulgidus Pelota reveals inter-domain structural plasticity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.237, 66.685, 98.380 |
| Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| Rwork | 0.209 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 17950 | |
| Completeness [%] | 96.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100mM imidazole pH 6.0, 200mM MgCl2, 8%(w/v) polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100mM imidazole pH 6.0, 200mM MgCl2, 8%(w/v) polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
