3OBW

Crystal structure of two archaeal Pelotas reveal inter-domain structural plasticity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-01
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	124.394, 34.329, 90.109
Unit cell angles	90.00, 103.64, 90.00

Refinement procedure

Resolution	15.000 - 2.600
Rwork	0.228
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.439
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.21)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.600
Number of reflections	10800
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	100mM imidazole pH 8.0, 1.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	8	297	100mM imidazole pH 8.0, 1.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 297K