3OBV
Autoinhibited Formin mDia1 Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-13 |
| Detector | SBC-3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.926, 208.519, 131.516 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.750 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.26100 |
| Structure solution method | MAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.054 | 0.541 |
| Number of reflections | 123292 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 95.4 | 60.5 |
| Redundancy | 3.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.75 | 293 | 10% PEG 1500, 25% sucrose, 0.1 M MES pH 6.75, 0.15 M NaCl, 1 mM DTT, vapor diffusion, hanging drop, temperature 293K |
