3OB8

Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.939
Spacegroup name	P 21 21 21
Unit cell lengths	140.381, 153.454, 217.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 2.800
R-factor	0.2155
Rwork	0.214
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.323	49.300	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.099	0.034	0.431
Total number of observations		46167	193533
Number of reflections	115849
<I/σ(I)>	24.5	18.9	1.9
Completeness [%]	100.0	99.4	100
Redundancy	11.9	11.8	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	25 % Polyethylen Glycol (PEG) 3350, 0.1 M BisTris pH 7.0, 0.2 M Sodium Tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K