3OAH
Structural Characterization of the Dual Glycan Binding Adeno-Associated Virus Serotype 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.917 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 263.000, 263.000, 609.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| Rwork | 0.275 |
| R-free | 0.28750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AAV1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | 0.607 |
| Number of reflections | 119617 | |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 72.3 | 50.5 |
| Redundancy | 2.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 25mM Tris-HCl pH7.5 with 60 mM MgCl2 and 100mM NaCl, 4% PEG6000, and 25%glycerol, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
