3OA8
Diheme SoxAX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.172, 74.629, 74.295 |
| Unit cell angles | 110.02, 108.08, 108.54 |
Refinement procedure
| Resolution | 35.310 - 1.770 |
| R-factor | 0.18529 |
| Rwork | 0.183 |
| R-free | 0.23044 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.454 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 111016 | |
| Completeness [%] | 93.7 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 3350, 0.2M lithium sulfate, 0.1M Tris-HCl pH 8.5, 0.01M spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 3350, 0.2M lithium sulfate, 0.1M Tris-HCl pH 8.5, 0.01M spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
