3O9O
Crystal Structure of GBS1074, an Esat-6 homologue from Group B Streptococcus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 76.220, 76.220, 151.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.080 - 2.000 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.21920 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.080 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 18255 |
| <I/σ(I)> | 34 |
| Completeness [%] | 99.7 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 291 | 0.75 M LiSO4, 0.6 M (NH4)2SO4, 0.1 M Na citrate, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
