3O97
Crystal Structure of the complex of C-lobe of lactoferrin with indole acetic acid at 2.68 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.234, 50.396, 65.893 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 63.240 - 2.680 |
| R-factor | 0.17456 |
| Rwork | 0.172 |
| R-free | 0.22243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ib1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.075 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 2.780 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Number of reflections | 10731 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 98.2 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, temperature 298K |
