3O8V

Crystal Structure of the Tudor Domains from FXR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97904
Spacegroup name	H 3
Unit cell lengths	71.857, 71.857, 94.055
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.930 - 2.500
R-factor	0.2201
Rwork	0.218
R-free	0.26120
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.760
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.074	0.067	0.549
Number of reflections	6291
<I/σ(I)>	19.1
Completeness [%]	99.9	99.2	100
Redundancy	3.9	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	291	1.4M Ammonium Sulfate, 0.1M HEPES pH 6.8, 0.25 M NaCl, 10 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 291K