3O7P
Crystal structure of the E.coli Fucose:proton symporter, FucP (N162A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.90922 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 119.180, 119.180, 99.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.302 - 3.196 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o7q |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.302 | 3.310 |
| High resolution limit [Å] | 3.196 | 3.200 |
| Rmerge | 0.065 | 0.699 |
| Number of reflections | 13809 | |
| <I/σ(I)> | 20.1 | 1.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 34% PEG 400, 0.1M cacodylate, 0.1M MgCl2, 0.05M NaF, 0.2M KCl, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
