3O7B
Crystal structure of Archaeoglobus Fulgidus Nep1 bound to S-adenosylhomocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.083, 70.083, 95.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.900 - 1.450 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 48192 | |
| <I/σ(I)> | 32.9 | 5.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.3 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 3-4M NaCl, 0.1M HEPES, pH 7.0-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
