3O77
The structure of Ca2+ Sensor (Case-16)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.499, 106.941, 43.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.010 - 2.350 |
| R-factor | 0.2015 |
| Rwork | 0.198 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.891 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.010 | 2.540 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 18555 | |
| <I/σ(I)> | 14.4 | 3.3 |
| Completeness [%] | 98.7 | 90.1 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | Reservoir: 50mM Imidazol, 1.9M Na2malonate pH 6.4; Protein stock solution: 50mM Tris HCl (pH 7.4), 150mM NaCl, 10mM dithiothreitol, protein 4.1mg/ml; Drop ratio reservoir/protein = 1/3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
