3O75
Crystal structure of Cra transcriptional dual regulator from Pseudomonas putida in complex with fructose 1-phosphate'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.711, 120.220, 61.860 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 14.944 - 2.300 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o74 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.050 |
| Total number of observations | 79179 |
| Number of reflections | 23340 |
| <I/σ(I)> | 12.6 |
| Completeness [%] | 99.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 0.1M Mes pH 6.5, 0.2M NaAc, 15 % PEG 8000, VAPOR DIFFUSION, temperature 294K |
