3O6M
Anti-Tat HIV 11H6H1 Fab' complexed with a 9-mer Tat peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.840, 69.720, 62.160 |
| Unit cell angles | 90.00, 109.94, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.400 |
| R-factor | 0.2146 |
| Rwork | 0.211 |
| R-free | 0.29031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mlb |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.925 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.354 |
| Number of reflections | 19524 | |
| <I/σ(I)> | 21.79 | 3.79 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | soaking | 6.5 | 289 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% (v/v) polyethylene glycol 550 monomethyl ether, Soaking of Fab' crystals with 5mM Tat-peptide for 15 min, soaking, temperature 289K |
