3O5Y
The Crystal Structure of the GAF domain of a two-component sensor histidine kinase from Bacillus halodurans to 2.45A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 96.961, 96.961, 104.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.990 - 2.450 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.339 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 5.280 | 2.450 |
Rmerge | 0.066 | 0.047 | 0.626 |
Number of reflections | 18880 | ||
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.6 | 97 | 100 |
Redundancy | 7.9 | 7.1 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 3350, 0.2M Magnesium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |