3O4W
Crystal structure of dimeric KlHxk1 in crystal form IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.710, 91.990, 92.580 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 29.200 - 1.610 |
| R-factor | 0.16992 |
| Rwork | 0.169 |
| R-free | 0.21996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o08 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.094 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 129734 | |
| <I/σ(I)> | 18.5 | 3.2 |
| Completeness [%] | 87.0 | 98.7 |
| Redundancy | 5.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 292 | 1MICROL RESERVOIR + 1MICROL PROTEIN, RESERVOIR: 2.5M AMMONIA SULFATE, 0.1M CHES PH 9.5, PROTEIN: 10MG/ML KLHXK1, 10MM TRIS PH 7.4, 1MM EDTA, 1MM DTT, 0.5MM PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
