3O3K

Crystal structure of AF1382 from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-12
Detector	MARRESEARCH
Wavelength(s)	1.9
Spacegroup name	P 42
Unit cell lengths	53.540, 53.540, 41.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.860 - 2.300
R-factor	0.232
Rwork	0.232
R-free	0.26800
Structure solution method	Sulfur SAD
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SGXPRO
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	5325
<I/σ(I)>	124.7	17.4
Completeness [%]	99.9	99.2
Redundancy	25	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	291	USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (2.8 MG/ML) AND SOLUTION CONTAING 0.1M SODIUM CITRATE/CITRIC ACID PH 5.5, 0.1M SODIUM CHLORIDE, 0.1M LITHIUM SULPHATE, 30% V/V PEG 400, Microbatch, temperature 291K