3O3I

Crystal Structure of human Hiwi1 PAZ domain (residues 277-399) in complex with 14-mer RNA (12-bp + 2-nt overhang) containing 2'-OH at its 3'-end

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 2 2
Unit cell lengths	44.303, 44.303, 147.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.801
R-factor	0.24494
Rwork	0.243
R-free	0.29701
Starting model (for MR)	Hiwi1(V361M)PAZ domain
RMSD bond length	0.005
RMSD bond angle	0.849
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.900
High resolution limit [Å]	2.800	5.980	2.800
Rmerge	0.096	0.049	0.361
Number of reflections	3966
<I/σ(I)>	14.8		1.9
Completeness [%]	96.6	95.4	78.7
Redundancy	4.1	3.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	1-5% PEG3350, 50 mM NaCl, 50 mM Na-acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K