3O3C
Glycogen synthase basal state UDP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Detector technology | CCD |
Collection date | 2009-08-01 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.01 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.586, 167.209, 121.251 |
Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
Resolution | 48.288 - 3.512 |
R-factor | 0.2209 |
Rwork | 0.219 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3naz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.114 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.570 |
High resolution limit [Å] | 3.510 | 9.510 | 3.510 |
Rmerge | 0.110 | 0.065 | 0.444 |
Number of reflections | 46381 | ||
<I/σ(I)> | 13 | ||
Completeness [%] | 99.0 | 99.5 | 92.7 |
Redundancy | 3.6 | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris pH 8.0, 20% PEG 3500, 200 mM LI2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |