3O2U
S. cerevisiae Ubc12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-03-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.451, 35.938, 87.035 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 25.921 - 2.003 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y8x Chain A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 23534 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 27% PEG 3350, 0.1M Tris, 0.2M NaCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
