3O1Z

High resolution crystal structure of histidine triad nucleotide-binding protein 1 (Hint1) double cysteine mutant from rabbit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-17
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 43 21 2
Unit cell lengths	39.790, 39.790, 142.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.170 - 1.300
R-factor	0.13002
Rwork	0.128
R-free	0.17612
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3LLJ
RMSD bond length	0.024
RMSD bond angle	2.039
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP (10.2.31)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.170	1.370
High resolution limit [Å]	1.300	1.300
Rmerge	0.103	0.680
Number of reflections	29103
<I/σ(I)>	14.2	4.1
Completeness [%]	99.4	99
Redundancy	12.4	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	30% PEG 8000, 0.1M sodium cacodylate pH 6.5, protein concentration 10 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 281K