3O1L

Crystal structure of a formyltetrahydrofolate deformylase (PSPTO_4314) from Pseudomonas syringae pv. tomato str. DC3000 at 2.20 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	131.271, 131.271, 102.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.728 - 2.200
R-factor	0.1745
Rwork	0.173
R-free	0.20230
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.433
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.728	29.728	2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge	0.085	0.029	0.826
Number of reflections	46149	8817	8829
<I/σ(I)>	12.71	37.8	1.9
Completeness [%]	99.8	99.4	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.57	293	1.4500M ammonium sulfate, 12.0000% Glycerol, 0.1M TRIS pH 8.57, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K