3NZN
The crystal structure of the Glutaredoxin from Methanosarcina mazei Go1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.423, 31.542, 57.935 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 51.690 - 1.100 |
| R-factor | 0.14907 |
| Rwork | 0.147 |
| R-free | 0.18928 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.637 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.690 | 1.129 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.072 | 0.489 |
| Number of reflections | 63391 | |
| <I/σ(I)> | 21.24 | 1 |
| Completeness [%] | 87.7 | 35.67 |
| Redundancy | 4.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M (NH4)2SO4, 20% PEG3350, 0.1M Na-hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
